PCR detection kit

Legionella

Detection of legionella using real-time PCR

Application:

Legionella belongs to a pathogenic group of gram-negative, non-spore forming bacteria and is prevalent in many environments, including soil and water systems. The industry requires a rapid and reliable analytical method for detection or monitoring to enable optimal management and control of Legionella in cooling water and process water systems. Conventional cultures are generally used to detect Legionella in water samples, but it takes up to 10 days to obtain a reliable result. In addition, the sensitivity of the culture is low, especially if the samples also contain microorganisms that inhibit Legionella growth and viable but non-culturable Legionella cells (VBNC) are not detected. To overcome these difficulties, we have developed a multiplex real time PCR method that can detect, differentiate and quantify Legionella species much earlier and independent of culture status.

In accordance with Directive (EU) 2020/21842 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption, a parameter value for legionella of < 1,000 CFU/L is required for the newly introduced risk assessment of drinking water installations.

GEN-IAL^® Legionella Differentiation PCR-Kit

Qualitative and quantitative PCR detection and differentiation of Legionella spp. and Legionella pneumophila in drinking water. The test kit used is developed and validated by GEN-IAL®. Detection is carried out by fluorescence measurement using the hydrolysis probe format (TaqMan^®). Using hot-start PCR plus double-labelled sequence-specific probes (FAM / HEX / ROX / CY5), a measurable fluorescence signal of a defined wavelength is emitted during the extension phase if hybridization to the target sequence is correct. An inhibition control is amplified together with the specific sequence in a reaction tube to exclude false negative results due to inhibition.