PCR detection kit

Beverage spoilage microorganisms

Detection of beverage spoiler using real-time PCR

Application:

The detection and identification of beverage spoilage microorganisms has so far been carried out using time-consuming cultural selective enrichment. Only after at least five days does a turbidity of the cultured sample indicate an infestation with beverage spoiler, often too late to be able to take countermeasures in time. The PCR method now offers beverage producers the possibility of reliably detecting contamination with microorganisms at any point in the production process within just a few hours. To prevent the transfer of harmful microorganisms into the product, both the raw materials used and the equipment and pipe systems used should be checked for microbial infestation at regular intervals.

QuickGEN PCR-Kits:

The test kits used are developed and validated by GEN-IAL®and include bacteria, yeasts and molds. Screening and specific PCR systems are used. The verification procedures are continuously adapted to market requirements. We will be happy to assist you in interpreting the analysis results on request.

Detection is performed by fluorescence measurement using the hydrolysis probe format (TaqMan®). Using hot-start PCR plus double-labelled sequence-specific probes (FAM / HEX / ROX / CY5 or ATTO 490LS), a measurable fluorescence signal of a defined wavelength is emitted during the extension phase if hybridization to the target sequence is correct. An inhibition control is amplified together with the specific sequence in a reaction tube to exclude false negative results due to inhibition.

Advantages:

• DNA extraction and PCR are performed simultaneously
• Result in a maximum of 1.5 hours
• Can be used for all product types (beverages, concentrates, yeast suspensions, etc.)
• Open PCR systems (no binding to PCR devices)
• Large selection of QuickGEN PCR-Kits (see product catalog)