DNA extraction kit

Simplex^® Easy Spin Legionella

Application:

Manual DNA extraction from drinking water, cooling water, waste water and sewage sludge using silica membrane technology

The background to this is the new Directive (EU) 2020/21842 of the European Parliament and of the Council of 16 December 2020 on the quality of water intended for human consumption with the first-time specification of a parameter value for the Legionella parameter of < 1,000 CFU/L for the newly introduced risk assessment of drinking water installations.

The test applies to hot water systems with a storage tank volume of 400 liters or more or with a pipe volume of more than 3 liters between the water heater and the distribution point.

The test frequency for systemic testing for legionella in a building water supply system is initially once a year when drinking water is supplied to the public. Inspection intervals of up to three years by the public health department are possible.

Principle:

The method is based on spin column technology. After chemical lysis of the cells, the DNA binds to a silica membrane. Cell residues, salts and inhibitors are removed by washing steps. The DNA is released from the silica membrane by heating in TE or H₂O. The kit is robust against strongly acidic or alkaline, contaminant-rich and difficult to isolate samples. The DNA is of high purity and is available for further molecular biological analyses (e.g. real-time PCR, sequencing….).